Measurement

Part:BBa_M30007

Designed by: natalie kuldell   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2005-12-04)


-- No description --

Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of P22 c2 gene. The P22 c2 protein represses expression of mRFP (M30002). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced. A second isolate from this construction was saved as M30008.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1626
    Illegal AgeI site found at 1738
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 300


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Categories
//classic/measurement/uncategorized
Parameters
None